Key features and details | |
Cat. No. | MABL-2468 |
Name | Anti-Nerve growth factor mAbs |
Clone No. | AFD-mab 911 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, crystallization, in vitro, in vivo, inhibition, therapeutic, ELISA |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | The antibody is specific for NGF. The NGF epitope is composed of β-strand segments from both NGF monomers. NGF is involved primarily in the growth, as well as the maintenance, proliferation, and survival of nerve cells (neurons). |
Alternative Name | Beta-nerve growth factor; Beta-NGF |
UniProt | P01138 |
Immunogen | |
Application Notes | The specificity of the original format of the antibody to NGF was confirmed by ELISA analysis (EC50 = 0.37 nM). The antibody was evaluated for its ability to block the binding of hNGF to the TrkA and p75 NGF receptors in various in vitro assays, such as blocking of TrkA autophosphorylation and blocking of NGF-dependent survival of dorsal root ganglion sensory neurons. The antibody was a potent blocker of all activities (Hongo et al., 2000; PMID: 10952410). In vivo administration of the antibody significantly reduces bone cancer pain behaviors (Sevcik et al., 2005; PMID: 15836976 and Halvorson et al., 2005; PMID: 16230406 and Buehlmann et al., 2019; PMID: 30161041) and fracture pain-related behaviors (Koewler et al., 2007; PMID: 17638576). |
Antibody First Published | Hongo et al. Antibody binding regions on human nerve growth factor identified by homolog- and alanine-scanning mutagenesis. Hybridoma. 2000 Jun;19(3):215-27. PMID:10952410 |
Note on publication | The original paper describes the generation and characterization of a panel of monoclonal antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
