Anti-Neisseria meningitidis group B capsular polysaccharide mAbs

Cat. No.

MABL-2466

Name

Anti-Neisseria   meningitidis group B capsular polysaccharide mAbs

Clone No.

AFD- HmenB3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

bactericidal, ELISA, FC

Species Reactivity

Escherichia coli, Neisseria meningitidis

Basic Information

Specificity

This antibody binds to the capsular   polysaccharide of both Neisseria meningitidis group B and E. coli K1. It also   reacts with EV36, a nonpathogenic E. coli K-12 strain

Alternative Name

CPS 6B; capsular PS B

UniProt

Immunogen

This antibody was raised by immunising   BALB/cByJ mice with E. coli K1 bacteria (strain RS218). The E. coli K1   capsular polysaccharide antigen shows structural and immunological identity   with the meningococcal group B antigen, as both are α(2-8)-linked p

Application Notes

The binding characteristics of this antibody have been assessed in flow cytometric analysis; this antibody binds to N.   meningitidis group B and E. coli EV36 (Shin et al, 2001). It does not react   with N. meningitidis groups A, C, and Y, and is non-autoreactive, as it does   not react with human neuronal α(2-8) polysialic acid (Shin et al, 2001). This   antibody has been used in a sandwich type ELISA to investigate the   cross-reactivity of bacteriophage clones displaying the antigenic properties   of serotype 6B pneumococcal capsular polysaccharide (Shin et al, 2002). In   the presence of rabbit and human complement, this antibody exhibits   bactericidal activity (Shin et al, 2001).

Antibody First   Published

Shin et al. Monoclonal antibodies specific   for Neisseria meningitidis group B polysaccharide and their peptide   mimotopes. Infect Immun. 2001 May;69(5):3335-42. PMID:11292756

Note on publication

Describes the original generation of this   antibody, and its characterisation in ELISA analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.