Key features and details | |
Cat. No. | MABL-2442 |
Name | Anti-Myosin mAbs |
Clone No. | AFD- 2D6-B1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for MF1, a fragment spanning amino acids 1 - 562 in the S1 domain of the head region of murine cardiac myosin. This antibody cross-reacts with keratin and GlcNAc-BSA. |
Alternative Name | Q02566; Q91Z83; Q61879; Q6URW6; O08638; P13542; P13541; P97434; A2AQP0; Q02566 |
UniProt | |
Immunogen | This antibody was raised by immunising DBA/2 mice with cardiac myosin purified from BALB/c mouse hearts. |
Application Notes | The reactivity of this antibody with mouse cardiac myosin and GlcNAc-BSA has been confirmed in ELISA analysis (Malkiel et al, 2000).The affinity of this antibody for myosin is higher than its affinity for GlcNAc-BSA (Malkiel et al, 2000). Administration of this antibody induces autoimmune myocarditis in DBA/2 mice, but not BALB/c mice (Liao et al, 1995). Equally, in immunohistochemical analysis, this antibody has been shown to be deposited throughout the hearts of DBA/2 mice, but not BALB/c mice (Liao et al, 1995). |
Antibody First Published | Liao et al. Antibody-mediated autoimmune myocarditis depends on genetically determined target organ sensitivity The Journal of Experimental Medicine, 1995, Vol.181(3), p.1123-1130 PMID:7869033 |
Note on publication | Describes the original generation of this antibody, its characterisation, and its administration to DBA/2 mice to induce autoimmune myocarditis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
