Key features and details | |
Cat. No. | MABL-2427 |
Name | Anti-MVEV mAbs |
Clone No. | AFD- M2-8E7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, NTRL, WB, ELISA |
Species Reactivity | Japanese Encephalitis Virus, Murray Valley encephalitis virus |
Basic Information | |
Specificity | This antibody reacts with domain III of the envelope protein of the Murray Valley encephalitis virus. The mAb also cross reacts with Japanese encephalitis virus. |
Alternative Name | Murray Valley encephalitis virus; envelope protein; E protein; Japanese encephalitis virus |
UniProt | P05769 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with structural and non structural viral proteins using a crude suspension of infected suckling mouse brain. |
Application Notes | This neutralizing antibody binds the the envelope protein of the Murray Valley encephalitis virues in the ectodomain III region. This mAb is reported to block haemagglutination. The competitive binding of the mAb to envelope protein was checked with ELISA (PMID:1703213). Recombinant MVEV E-DIII proteins were detected by M2-8E7 in a western blot assay (PMID: 29083957). This antibody is also reported to be used in immunoprecipitation for the identification of E proteins from cell lysates (PMID: 9770429, 9499070). |
Antibody First Published | Hall et al. Epitope analysis of the envelope and non-structural glycoproteins of Murray Valley encephalitis virus J Gen Virol (1990);71 ( Pt 12):2923-30. PMID:1703213 |
Note on publication | Describes the spatial arrangement, conformation, viral specificity and biological properties of epitopes recognized by a panel of MAbs produced to the E and NS1 proteins of MVEV. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
