Anti-Mouse antibody kappa light chain mAbs

Cat. No.

MABL-2389

Name

Anti-Mouse antibody kappa light chain mAbs

Clone No.

AFD- OX-20

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, FC

Species Reactivity

Mouse

Basic Information

Specificity

OX-20 binds specifically to mouse kappa   light chain of immunoglobulins. Kappa light chains are found in 95% of mouse  Igs with lambda light chains being found in other antibodies. Kappa and   lambda chains differ slightly in amino acids residue composition.

Alternative Name

kappa Lc; OX20; MRC OX-20; MRC OX20

UniProt

P01837

Immunogen

OX-20 was generated by hyperimmunizing rats   with mouse F(ab')2 generated by enzymatic digest.

Application Notes

OX-20 can be used for WB, IP and FC studies.

Antibody First   Published

Mason et al. The rat mixed lymphocyte  reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets   defined by monoclonal antibodies. Immunology 44:75-87 1981 PMID:6456222

Note on publication

Describes how rat anti-mouse antibodies were   shown to bind specifically to IgGs and not to IgM by indirect   haemagglutination of sheep red blood cells which had been coated with   different mouse mAbs specific for sheep red blood cells, and to mouse IgGs and not IgG molecules derived from other species (some cross reaction with   rabbit and guinea pig) (Galfre et al. 1979).

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.