Key features and details | |
Cat. No. | MABL-2347 |
Name | Anti-Mesothelin mAbs |
Clone No. | AFD- MB |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, WB, ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody can recognize and bind human mesothelin. This antibody binds a different antibody on the human mesothelin than another antibody called MN-1. Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)-linked cell surface protein highly expressed in several types of malignant tumors sometimes in association with increased tumor aggressiveness and poor clinical outcome. The membrane-anchored forms of this molecule may play a role in cellular adhesion. |
Alternative Name | MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; MN |
UniProt | Q13421 |
Immunogen | The original antibody was generated in mesothelin-deficient mice by immunization with plasmid cDNA corresponding to the mesothelin extracellular domain followed by a single boost with a recombinant human mesothelin-rabbit IgG Fc fusion protein. |
Application Notes | The binding characterization of this antibody was done using ELISA and SPR. The binding affinity of the original IgG2a antibody for mesothelin-Fc protein was determined by BIACore and reported to be Kd = 0.6 nM. This antibody can recognize membrane bound mesothelin on the surface of mesothelin positive H226, A1847, Hela and Panc3.014 cells (mesothelin-positive) in a flow cytometry. This antibody can also bind denatured human mesothelin in a western blot assay. This antibody was also used in the immunohistochemical staining of cancerous tissue like ovarian adenocarcinoma and normal tissues like peritoneal mesothelium on uterus, pleural mesothelium on lung and fallopian tube luminal fluid (PMID: 16115924). This antibody was used in the development of an ELISA for detection of serum mesothelin levels in healthy volunteers and random hospitalized patients with mesothelioma and ovarian cancer (PMID: 16428485). |
Antibody First Published | Onda et al. New monoclonal antibodies to mesothelin useful for immunohistochemistry, fluorescence-activated cell sorting, Western blotting, and ELISA. Clin Cancer Res. 2005 Aug 15;11(16):5840-6. PMID:16115924 |
Note on publication | Describes the generation of two antibodies against different epitopes of human mesothelin |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
