Key features and details | |
Cat. No. | MABL-2322 |
Name | Anti-mammalian sTnI mAbs |
Clone No. | AFD- 6G1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | cattle, Deer, Horse, Pig, Sheep |
Basic Information | |
Specificity | This antibody is specific for mammalian skeletal Troponin I (sTnI) and can cross-react with skeletal Troponin C (sTnC). |
Alternative Name | Skeletal Troponin I; Troponin I; skeletal Troponin C; sTnC |
UniProt | |
Immunogen | The original antibody was generated by immunizing BALB/c mice with heat-treated TnI in Freund's complete adjuvant, followed by two booster injections with TnI in Freund's incomplete adjuvant. |
Application Notes | This antibody was originally reported to react with equine (horse) Troponin I (TnI) as determined by ELISA (US7297500B2). Later, it was shown through WB to bind mammalian skeletal TNI (sTnI), specifically bovine (cow) and porcine (pig), where it was shown to be immunoreactive to both sTnI and skeletal Troponin C (sTnC). However, sTnC was not detected by this antibody following extraction using urea and βME supplemented extraction buffer, indicating that their addition leads to the destruction of the antibody's epitope on sTnC. In a sandwich ELISA, it was determined that this antibody detects heated bovine, cervine (deer), equine, ovine (sheep), and porcine samples (Jiang et al., 2020; DOI: 10.1016/j.foodcont.2019.107045). |
Antibody First Published | Hsieh et al. Use of troponin I as a species marker protein for meat speciation in both raw and heat-processed products. US7297500 |
Note on publication | The original publication describes the development and applications of monoclonal antibodies which bind to heat-treated proteins of meat. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
