Key features and details | |
Cat. No. | MABL-2277 |
Name | Anti-LPS mAbs |
Clone No. | AFD- CK3 (BP1 7F7) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Burkholderia pseudomallei, Burkholderia thailandensis |
Basic Information | |
Specificity | This antibody binds the surface lipopolysaccharide of Burkholderia pseudomallei and Burkholderia thailandensis. Burkholderia pseudomallei (BP), is Recently, BP has been recognized as a major cause of community-acquired septicemia, resulting in significant mortalitythe causative agent of melioidosis (Whitmore’s disease), is a gram-negative, facultative anaerobic, motile bacillus commonly found in the soil and stagnant waters. BP infection is often due to either direct inoculation into wounds and skin abrasions or inhalation of contaminated materials. Recently, BP has been recognized as a major cause of community- acquired septicemia, resulting in significant mortality. |
Alternative Name | Lipopolysaccharide; Burkholderia spp; Burkholderia pseudomallei; BP; Burkholderia thailandensis, melioidosis; Whitmore’s disease |
UniProt | |
Immunogen | The original antibody was generated by immunizing mice with various antigen preparations from B. pseudomallei (ATCC 23343). |
Application Notes | This antibody can be used for the detection of heat killed, sodium periodate treated, proteinase K treated or 0.02% SDS treated Burkholderia pseudomallei and Burkholderia thailandensis in a sample using ELISA. It was reported that this antibody could recognize 34, 38∼52 kDa polypeptide antigens from Burkholderia pseudomallei and 34, 38∼60 kDa polypeptide antigens from Burkholderia thailandensis in a western blot assay (PMID: 21573027). |
Antibody First Published | Yong-Kim et al. Production and characterization of chimeric monoclonal antibodies against Burkholderia pseudomallei and B. mallei using the DHFR expression system. PLoS One. 2011 May 9;6(5):e19867. PMID:21573027 |
Note on publication | Describes the generation and characterization of chimeric antibodies against Burkholderia pseudomallei and B. mallei. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
