Key features and details | |
Cat. No. | MABL-2261 |
Name | Anti-Lipoprotein lipase mAbs |
Clone No. | AFD- 5D2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, ICC, neutralizing, SPR, WB, ELISA, IF |
Species Reactivity | Bovine, Cat, Chicken, Guinea Pig, Rat, Human |
Basic Information | |
Specificity | The antibody is specific for lipoprotein lipase (LPL) and it binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The antibody does not cross react with mouse LPL. LPL is a key enzyme in triglyceride metabolism, it catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. |
Alternative Name | LPL; Phospholipase A1; EC 3.1.1.34 |
UniProt | P06858 |
Immunogen | The original antibody was generated by immunizing mice with bovine LPL. |
Application Notes | LPL was immunoprecipitated from postheparin plasma using this antibody. The antibody was used both to capture LPL and to detect the bound LPL in a sandwich ELISA, indicating the epitope to be present in duplicate. In another sandwich ELISA analysis, the antibody was used to detect the LPL antigen (Peterson et al., 1992, PMID: 1279089). The antibody detected LPL from human, chicken and guinea pig by western blot analysis. The specificity of the original format of the antibody was confirmed by ELISA analysis (Chang et al., 1998; PMID: 9831623). Pretreatment of LPL with the antibody totally suppressed LPL-induced monocyte adhesion to endothelial cells (Mamputu et al., 1997; PMID: 9323582). Further, LDL was immunoneutralized with the antibody, totally suppressing LPL-induced VSMC proliferation (Mamputu et al., 2000; PMID: 11031206). |
Antibody First Published | Peterson et al. Human lipoprotein lipase: relationship of activity, heparin affinity, and conformation as studied with monoclonal antibodies J Lipid Res. 1992 Aug;33(8):1165-70.PMID:1279089 |
Note on publication | The paper describes the generation and characterization of the antibody. The antibody is used to investigate how conformational changes in lipoprotein lipase affect its molecular functions. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
