Key features and details | |
Cat. No. | MABL-2256 |
Name | Anti-LHRH mAbs |
Clone No. | AFD- SMI41 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IHC-P, RIA, therapeutic, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | SMI41 reacts with LHRH with an affinity of 10 pM and is specific for its C-terminal pentapeptide (G-L-R-P-G). |
Alternative Name | GnRH; LHRH; LNRH; Anti-Gonadotropin-Releasing Hormone; GNRH1; Gonadoliberin; Gonadorelin; luteinizing hormone release hormone; SMI-41; SMI 41 |
UniProt | P30968 |
Immunogen | LHRH diacetate tetrahydrate was conjugated to potato acid phosphatase and an emulsion composed of the conjugate and Freunds complete adjuvant was injected into the hind limb of female CAF1 mice. Immune spleen cells were fused with P3-X63-Ag8.653/3 myeloma cells by a modification of the original cell fusion method using polyethylene glycol. A hybridoma screening assays were used to select for high affinity monoclonal antibodies. |
Application Notes | SMI-41 has been used in immunocytochemistry experiments to detect the expression of mature LHRH in the brain detect cells containing mature LHRH peptide (Avigdor 2004). SMI-41 also has been expressed in mice via injection of a replication-incompetent, recombinant adeno-associated virus designed to express the antibody. This has been used to cofer long-term infertility in male and female mice (Li 2015). |
Antibody First Published | Knapp and Sternberger High Affinity Monoclonal Antibodies to Luteinizing Hormone-releasing Hormone J Neuroimmunol. 1984 Aug;6(5):361-71. PMID:6378971 |
Note on publication | Describes the generation of monoclonal antibodies against Gonadotropin-releasing hormone. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
