Key features and details | |
Cat. No. | MABL-2232 |
Name | Anti-Lambda light chain mAbs |
Clone No. | AFD- N10/2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for lambda light chains and does not show cross-reactivity with kappa light chains. It stains B cell follicles in human lymphoid tissues. Lambda light chain is one of the two types of light chains of mammalian immunoglobulins (one Ig has only one type of chains). Usually, the ratio of kappa to lambda is 70:30 but it might be disturbed in various B-cell conditions, such as myeloma. |
Alternative Name | IGL; immunoglobulin lambda light chain; lambda chain; lambda; λ immunoglobulin light chain; anti-λ |
UniProt | P0DOY2 |
Immunogen | This antibody was raised by immunising BALB/c mice with purified human IgG. |
Application Notes | N10/2 antibody may be utilised in the identification of certain pathologies (mainly associated with B-cells), such as leukemias, plasmacytomas, and some non-Hodgkin's lymphomas. For example, one group used this antibody during an immunohistochemical characterisation of human fetal bone sections engrafted with Waldenström macroglobulinemia cells (Tassone et al., 2005). Another group utilised N10/2 antibody to analyse the light chains expression in post-transplant lymphoproliferative disorders via immunohistochemistry on formalin-fixed, paraffin-embedded tissue samples (Capello et al., 2006). |
Antibody First Published | Mason et al. Double immunofluorescence labelling of routinely processed paraffin sections. J Pathol. 2000 Aug;191(4):452-61. PMID:10918222 |
Note on publication | This article describes the earliest found application of this antibody where it was successfully utilised in immunohistochemical labelling. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
