Key features and details | |
Cat. No. | MABL-2227 |
Name | Anti-LAM mAbs |
Clone No. | AFD- BJRbL03 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA, FC |
Species Reactivity | Mycobacterium bovis, Mycobacterium tuberculosis |
Basic Information | |
Specificity | This antibody is specific for the LAM protein of Mycobacterium tuberculosis but shows cross- reactivity with other Mycobacterium species like M. bovis. M. tuberculosis causes the disease tuberculosis. |
Alternative Name | Lipoarabinomannan; Mycobacterium cell wall polysaccharide |
UniProt | |
Immunogen | This antibody was generated by immunizing a rabbit with Mycobacterium tuberculosis H37Rv cell wall. |
Application Notes | The ability of the rabbit version of this antibody to bind LAM was determined using ELISA on LAM. Furthermore, the affinity of this antibody to LAM was determined using a ForteBio Octe machine. Finally, the ability of this antibody to distinguish isolates of M. tuberculosis (MTB), M. avium- intracellulare (MAI), M. abscessus, and M. fortuitum-abscessus (MA-MF) was assessed using flow cytometry; it bound all bacteria (Yan et al., 2021; PMID: 32146163). This antibody was used as a capture antibody in a sandwich ELISA (Yan et al., 2023; PMID: 37764103). |
Antibody First Published | Yan et al. Generation of mycobacterial lipoarabinomannan-specific monoclonal antibodies and their ability to identify mycobacterium isolates. J Microbiol Immunol Infect. 2021 Jun;54(3):437-446. PMID:32146163 |
Note on publication | Novel rabbit monoclonal antibodies (mAbs) against LAM for immunodetection purposes were developed. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
