Key features and details | |
Cat. No. | MABL-2217 |
Name | Anti-LAG-3 mAbs |
Clone No. | AFD- Ab835 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for human LAG-3. The antibody does not cross react with cynomolgus LAG-3. |
Alternative Name | CD223; LAG3; FDC; Lymphocyte activation gene 3 protein |
UniProt | P18627 |
Immunogen | |
Application Notes | The antibody is a humanized version of the mouse antibody mab7c. The specificity of the original format of the antibody was confirmed by ELISA analysis (EC50=0.12 nM). The binding activity of the humanized antibody was tested using the hLAG-3 positive cell line, and the results showed that the antibody was positive for hLAG-3. The binding activity of the antibody and hLAG-3 positive cells was EC50 =0.41 nM. The binding affinity of the antibody to hLAG-3 was measured by surface plasmon resonance (Kd= 0.52 nM). The antibody blocked the binding of MHCII and human LAG-3 with IC50 = 2.27 nM and it prevented LAG-3 and MHCII from binding to activate human T cell activity (release IL2) (EC50=1.74ug/ml) (CN110172099). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
