Key features and details | |
Cat. No. | MABL-2200 |
Name | Anti-Keratin mAbs |
Clone No. | AFD- IAD7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ISB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for keratin with an increased affinity for native keratin over sulfur mustard-exposed (SM-exposed) keratin. |
Alternative Name | |
UniProt | |
Immunogen | The original antibody was selected from existing phage display libraries. |
Application Notes | The binding characteristics of this antibody were observed in an ELISA and immunoslotblot (ISB) assay. Increased exposure levels of keratin resulted in a decreased emission, indicating decreased stability of the antibody-keratin interactio; the formation of adducts or crosslinks disrupts effective antibody binding (Bikker et al., 2007; PMID: 17539823). |
Antibody First Published | Bikker et al. Detection of sulfur mustard adducts in human callus by phage antibodies Chem Biol Drug Des. 2007 May;69(5):314-20. doi: 10.1111/j.1747-0285.2007.00504.x PMID:17539823 |
Note on publication | The original publication explores the application of phage display in the detection of sulfur mustard adducts in human callus, demonstrating the successful use of antibody phage display for immunodiagnostics of a chemical warfare agent. Furthermore, the publication describes the generation and characterization of clone IAD7. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
