Anti-kappa light chain mAbs

Cat. No.

MABL-2184

Name

Anti-kappa light chain mAbs

Clone No.

AFD- NH3/41.34

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB

Species Reactivity

Human

Basic Information

Specificity

This antibody recognises human immunoglobulin   kappa light chain. The antibody does not cross react with rhesus macaque   IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4.

Alternative Name

kappa Lc; human immunoglobulin kappa light   chain; kappa chain; immunoglobulin kappa light chain; NH3/41

UniProt

Immunogen

This antibody was raised by immunising Balb/c mice with whole IgG fraction and subsequent hybridoma cells formation.

Application Notes

NH3/41.34 antibody detects kappa light chains   from a human IgGs and Bence-Jones protein samples via Western blot under reducing conditions (Thorpe et al., 1984). This antibody is equally able to   detect full IgGs via Western blot when the samples are transferred from the   non-reducing SDS polyacrylamide gels (Thorpe et al., 1984). Further, this   antibody was capable of detecting IgGs via Western blot from native gels run   under acidic conditions (Thorne et al., 1984). Finally, the specificity of   the antibody for human IgG1 was confirmed by ELISA analysis. No cross   reactivity with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus   monkey IgG4 was detected.

Antibody First   Published

The use of monoclonal antibodies to human   IgG in blood transfusion serology. Biotest Bulletin 4: 348-352 (1983)

Note on publication

This article describes the generation and   characterisation of the NH3/41.34 antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.