Anti-Integrin α-M mAbs

Cat. No.

MABL-2161

Name

Anti-Integrin α-M mAbs

Clone No.

AFD-107

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds to the MIDAS face of the   A domain from integrin CD11b.

Alternative Name

CD11b; CD11 antigen-like family member B;   CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; leukocyte   adhesion receptor MO1; Neutrophil adherence receptor; complement receptor 3

UniProt

P11215

Immunogen

Wild type (WT) recombinant CD11b.

Application Notes

The antibody binds specifically to integrin α-M,   a protein subunit of the α-M- β-2 molecule, which is expressed on the surface   of leukocytes. Integrin α-M is involved in the adhesive interaction between   molecules of the innate immune system, and plays a role in the uptake of   complement- coated particles. The monoclonal antibody 107 has a blocking   activity by binding to the metal-ion- dependent-adhesion site, favouring a   low affinity conformation of the antigen.

Antibody First   Published

Li R, Haruta I, Rieu P, Sugimori T, Xiong   JP, Arnaout MA. Characterization of a conformationally sensitive murine   monoclonal antibody directed to the metal ion-dependent adhesion site face of   integrin CD11b. J Immunol. 2002 Feb 1;168(3):1219-25. PMID:11801658

Note on publication

Describes the binding of the murine   monoclonal antibody 107 to integrin CD11b. The antibody mimics the binding of   divalent cations to the integrin, thereby inhibiting the association of CD11b   to 11bA, blocking phagocytosis by human neutrophils.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.