Anti-Integrin beta-3 mAbs

Cat. No.

MABL-2158

Name

Anti-Integrin beta-3 mAbs

Clone No.

AFD- Y2/51

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FACS, WB, IF, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody recognises integrin beta-3   which is a receptor for multiple ligands, such as laminin, fibronectin,   prothrombin and others. It is involved in platelet-platelet interactions   leading to a blood clot creation. Mutations in this protein are known to be a   cause of Glanzmann thrombasthenia, the most common genetic disease of   platelets.

Alternative Name

Integrin b3; Platelet membrane glycoprotein   IIIa; GPIIIa; CD61; GP3A; INGRB3; ITG B3; ITGB3; Integrin β3

UniProt

P05106

Immunogen

This antibody was raised by immunising   Balb/c mice with PHA-stimulated peripheral blood cells and subsequent fusing   spleen cells and NSl myeloma cells.

Application Notes

This antibody has been extensively used as a   marker of megakaryocytes. It is very effective in the immunohistochemical   detection of platelets and megakaryocytes in formalin-fixed tissue samples as   well as via Western blot and was also recommended for the identification of   blood clots in blood vessels (Gatter et al., 1988). Y2/51 antibody has been   also found useful in characterisation of other cells and tissues. For   instance, one group used it (in concert with other antibodies) to compare   immunohistochemically fibroblasts from normal and ruptured human anterior   cruciate ligaments for tissue engineering purposes (Brune et al., 2007).   Finally, another group performed immunofluorescence staining of HCT15 cells   with this antibody and noted increased Integrin beta-3 expression in   butyrate-preselected cells in a study on the acquisition of   butyrate-resistance by colon cancer cells (Serpa et al., 2010).

Antibody First   Published

Gatter et al. The immunohistological   detection of platelets, megakaryocytes and thrombi in routinely processed   specimens. Histopathology. 1988 Sep;13(3):257-67. PMID:3192191

Note on publication

This article describes generation and   characterisation of Y2/51 antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.