Key features and details | |
Cat. No. | MABL-2138 |
Name | Anti-Insulin mAbs |
Clone No. | AFD- Ab 123 (mAb3, CC9C10) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA, ELISA, FC |
Species Reactivity | calf, rodent, Human |
Basic Information | |
Specificity | This antibody binds an epitope on the B chain of calf, human and rodent insulin. Insulin is a peptide hormone produced by the beta cells of the pancreatic islets. It reduces blood glucose concentration by promoting its absorption from the blood into liver, fat and skeletal muscle cells. Decreased or absent insulin activity results in diabetes mellitus, a condition of high blood sugar level (hyperglycemia). |
Alternative Name | INS; proinsulin |
UniProt | P01317 |
Immunogen | The original antibody was generated by immunizing female BALB/c mice with calf insulin in Freund's complete adjuvant. |
Application Notes | This antibody can be used to detect insulin in a sample using ELISA and radioimmunoassay (PMID: 2109009; 6193965). This antibody was used in the flow cytometric analysis of insulin occupied B cell receptors in VH125SD B6 mice (PMID: 26109644). |
Antibody First Published | Schroer et al. Mapping epitopes on the insulin molecule using monoclonal antibodies. Eur J Immunol. 1983 Sep;13(9):693-700. PMID:6193965 |
Note on publication | Describes the generation of 18 different antibodies to map the antigenic determinants on the human insulin. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
