Key features and details | |
Cat. No. | MABL-2128 |
Name | Anti-IL4 mAbs |
Clone No. | AFD- 6D9G1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, neutralizing, WB, ELISA, FC |
Species Reactivity | Chicken |
Basic Information | |
Specificity | This antibody binds specifically to chicken IL-4, with no cross reactivities with human or mouse IL-4 and other chicken cytokines. |
Alternative Name | Interleukin 4; IL-4; chIL-4; chicken IL-4; B-cell stimulatory factor 1; Lymphocyte stimulatory factor 1 |
UniProt | Q5W4U1 |
Immunogen | The original antibody was raised by immunising BALB/C mice with recombinant chicken IL-4. |
Application Notes | The interleukin 4 is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. The antibody was used to explore the functionality of chicken IL-4. The specificity of this antibody (IgG2a) was confirmed in ELISA analysis (Chaudhari et al, PMID: 30459004). The antibody detected the recombinant chIL-4 by western blot. Similarly, the antibody detected chIL-4 production in HD11 cells by immunocytochemistry. This antibody was also used in flow cytometry analysis. The antibody was used in in vitro neutralization assay using HD11 cells and primary monocytes (Chaudhari et al, 2018; pmid: 30459004). |
Antibody First Published | Chaudhari et al. Interleukin‐4 (IL‐4) may regulate alternative activation of macrophage-like cells in chickens: A sequential study using novel and specific neutralizing monoclonal antibodies against chicken IL‐4. Vet Immunol Immunopathol. 2018 Nov;205:72-82. PMID:30459004 |
Note on publication | The article describes the generation of the antibody, and its use in investigating the functionality of chicken IL-4. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
