Key features and details | |
Cat. No. | MABL-2125 |
Name | Anti-IL2R mAbs |
Clone No. | AFD- AHT54 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | AHT54 recognises a determinant close to the IL-2 binding site. |
Alternative Name | CD25; Interleukin 2 receptor; IL-2R; IL2 receptor; IL-2 receptor; Tac antigen; p55; AHT-54; AHT 54 |
UniProt | P01589 |
Immunogen | BALB/c mice were immunized with human T lymphoblasts and spleen cells of the immunized animals were fused with X63-Ag8.653 mouse myeloma cells. The supernatants of the resultant hybridomas were screened by a proliferation inhibition assay (inhibition of the human T lymphoblast response to IL-2). Two of those (the clones AHT54 and AHT107) that produced antibodies which inhibited IL-2 dependent proliferation of human T lymphoblasts were selected, cloned and recloned. |
Application Notes | AHT54 has been used in ELISAs for the detection of soluble human IL-2R in response to different forms of helminthic and protozoal infections (O. Josimovic et al. 1988). AHT54 has also been shown to have anti-proliferative activities, and chimeric AHT54 antibodies with a human contant region have an increased inhibitory effect on proliferation of T lymphocytes (Rose 1992). |
Antibody First Published | Osawa H et al. Studies on the formation of high-affinity IL-2 binding sites of an IL-2 receptor p55 + p75 heterodimeric complex: functional importance of a determinant on the p55 subunit defined by a monoclonal antibody AHT-107 Immunology Letters, 20 (1989) 205 - 212 Elsevie PMID:2469650 |
Note on publication | Describes the of binding monoclonal antibodies AHT107 and AHT54 to IL-2R and their inhibitory effect on IL-2 dependent proliferation of human T lymphoblasts. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
