Key features and details | |
Cat. No. | MABL-2097 |
Name | Anti-IL-6 mAbs |
Clone No. | AFD- mAB1339 (OP-R003-1) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, therapeutic, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes and binds the interleukin-6 at amino acid residues that are crucial for binding to human IL-6R. The binding of this antibody blocks or inhibits the binding between human IL6 and human IL-6R. |
Alternative Name | IL6; IFNB2; BSF-2; CDF; Interleukin-6; IL-6; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2; IFN-beta-2 |
UniProt | P05231 |
Immunogen | The original antibody was generated from its murine parent antibody B-E8 by creating sunstiutional variants and their affinity maturation using phage display techniques |
Application Notes | This high affinity, fully human antagonistic monoclonal antibody mAb 1339 or OP- R003-1 was structurally derived from elsilimomab (B-E8, a murine anti-IL-6 mAb). In vitro studies demonstrated that mAb 1339 shares similar biological properties, including affinity and epitope specificity with its “parent” murine antibody. mAb 1339 detected human and monkey IL-6 but not murine or rat IL-6. No binding was observed with other IL-6 superfamily members. The binding affinity of this antibody to IL-6 was tested using ELISA. This fully human mAb inhibited the binding of IL-6 to its receptor as evidenced by flow cytometry using U266 cells and also inhibited IL-6 induced cell proliferation in murine B9, human TF1 or U266 cells (Vermot-Desroches et al., 2007). |
Antibody First Published | Vermot-Desroches et al. In Vitro Characterization of OP-R003-1, a New Fully Human Anti-IL6 Antibody. Blood (2007); 110 (11); 4522. |
Note on publication | Describes the in vitro characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
