Key features and details | |
Cat. No. | MABL-2088 |
Name | Anti-IL-6 mAbs |
Clone No. | AFD- 1.26.4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralizing, WB, ELISA |
Species Reactivity | Chicken |
Basic Information | |
Specificity | This antibody binds specifically to chicken IL-6, an interleukin that acts as both a pro- inflammatory cytokine and an anti-inflammatory myokine (cytokine produced from muscle). Further, the antibody shows cross-reactivity with human and murine IL-6. |
Alternative Name | Interleukin 6; Interleukin-6; ChIL-6; chicken IL-6 |
UniProt | Q90YI0 |
Immunogen | The original antibody was raised by immunising BALB/C mice with recombinant chicken IL-6. |
Application Notes | The specificity of this antibody (IgG2b) towards the native protein chIL-6 was confirmed in ELISA analysis (Scott et al, 2006; pmid: 16930722). The antibody detected the denatured chIL- 6 by western blot. The antibody showed inhibitory activity on murine IL-6 on a B9 hybridoma cell bioassay. The bioassay was run with varying concentrations of the antibody in order to determine the effective concentration for neutralizing IL-6. Further, recombinant forms of chicken, human and murine IL-6 were all tested in the bioassay. The antibody was effective in neutralizing all three recombinant IL-6 proteins. (Scott et al, 2006; pmid: 16930722). |
Antibody First Published | Scott et al. Monoclonal antibodies against chicken interleukin‐6. Vet Immunol Immunopathol. 2006;114(1‐2):173‐177 PMID:16930722 |
Note on publication | The paper describes the generation and characterization of the antibody, which is specific for chIL-6. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
