Key features and details | |
Cat. No. | MABL-2058 |
Name | Anti-IL-2 mAbs |
Clone No. | AFD- Jes6-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, NTRL, ELISA |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds murine IL-2. |
Alternative Name | Interleukin-2; IL2; T cell growth factor; JES6-1A12 |
UniProt | P04351 |
Immunogen | Rat antibodies were raised against murine IL-2. |
Application Notes | JES6-1 is a scFv reformat of the variable domain sequences of the JES6-1 monoclonal antibody (Spangler et al, 2015). It was shown in this study that JES6-1 scFv binding to mouse IL-2 would sterically hinder interaction with the IL-2 receptor, thereby blocking downstream signalling functions. This antibody is well known as a neutralising antibody against mouse IL-2, and is useful for cytokine assays such as ELISA (Abrams et al, 1992). |
Antibody First Published | Abrams et al. Strategies of Anti-Cytokine Monoclonal Antibody Development: Immunoassay of IL-10 and IL-5 in Clinical Samples Immunological Reviews 1992, No. 127 PMID:1387110 |
Note on publication | Used the anti-mouse IL-2 clone JES6-1A12 to assay the presence of mouse IL-2 in enzyme-linked assays. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
