Key features and details | |
Cat. No. | MABL-2033 |
Name | Anti-IL-17 mAbs |
Clone No. | AFD- Antibody 7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, functional assays, in vivo, SPR, ELISA |
Species Reactivity | Dog, Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody is specific for IL-17; it binds residues 74 (Leu), 85 (Tyr), 86 (H is), and 87 (Met) of the mature IL-17 sequence. In humans, It has been shown to bind both IL-17A and IL-17F. |
Alternative Name | IL17; IL-17A; IL17A; CTLA8; Interleukin-17A; IL-17A; Cytotoxic T- lymphocyte-associated antigen 8; CTLA-8; IL17F; IL-17F; Interleukin-17F; IL-17F; Cytokine ML-1 |
UniProt | |
Immunogen | The original antibody was produced by immunizing Balb/c mice with purified recombinant human IL-17. |
Application Notes | The IC50 values for this antibody as measured in an HT1080 assay in response to 1 nM human or cynomolgus monkey (cyno) IL-17A were 0.8 ± 0.2 nM and 4.8 ± 1.6 nM, respectively. Partial inhibition was also observed with IL-17F. Further assays showed cross-reactivity with dog IL-17A. This antibody neutralized the synergistic IL-6 response as measured in an altered human HT1080 assay with an IC50 value of 0.24 ± 0.05 nM. pA2 = 9.23 and Kd = 663 pM were measured when testing the affinity of this antibody for human IL-17A. Further kinetic analysis on recombinant human IL-17A (E. coli), cyno IL-17A (E. coli), and human IL-17A-Flag-His10 (HEK) yielded a 1:1 Langmuir KD and Bivalent Analyte KD of 0.195 nM, 0.512 nM, and 0.171 nM, and 2.098 nM, 1.340 nM, and 0.41-1.88 nM, appropriately. The biological activity of this antibody was tested extensively in the air pouch of Balb/c; it effectively inhibited IL-17A-induced IL-6 release and the influx of white blood cells in the air pouch, as reflected by low ID50 values. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
