Key features and details | |
Cat. No. | MABL-2027 |
Name | Anti-IL-13 mAbs |
Clone No. | AFD- 6A1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibition, neutralization, SPR, ELISA |
Species Reactivity | Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody is specific for human IL-13. It cross-reacts with cynomolgus monkey IL-13. |
Alternative Name | IL13; Interleukin-13; P600 |
UniProt | P35225 |
Immunogen | The original antibody was generate by immunizing SJL mice with E. Coli-produced recombinant human IL-13. |
Application Notes | The original format of this antibody (mouse IgG1) showed binding activity against E.coli-expressed recombinant human IL-13 in a sandwich ELISA with an EC50 of 0.049 and 0.039 µg/ml for human and cynomolgus IL-13. The antibody also exhibited potent binding and neutralization activities against various forms of IL-13. In inhibition assays, the antibody showed IC50 values of 0.165 µg/ml for blocking human IL-13 binding to IL-13Rα1 and 0.056 µg/ml for IL-13 binding to IL-13Rα2. Moreover, it demonstrated strong neutralization capabilities with ND50 (neutralizing dose) values of 0.0783 µg/ml for human IL-13 and 0.04 µg/ml for cynomolgus IL-13, indicating its efficacy in inhibiting IL-13-induced TF-1 cell proliferation (where the ND50 value is the concentration of monoclonal antibody required to reduce TF-1 cell proliferation by 50%, in response to a set concentration of IL-13). |
Antibody First Published | |
Note on publication | The original publication describes the generation and characterization of an anti- human IL-13 antibody and its derivatives, including its humanization. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
