Key features and details  | |
Cat. No.  | MABL-2010  | 
Name  | Anti-IgM1gj mAbs  | 
Clone No.  | AFD- TG4  | 
From  | Recombinant Antibody  | 
Isotype  | Engineer antibody  | 
Application  | ELISpot, IP, IHC  | 
Species Reactivity  | Nurse Shark  | 
Basic Information  | |
Specificity  | This antibody is specific for IgM1gj.  | 
Alternative Name  | |
UniProt  | |
Immunogen  | |
Application Notes  | This antibody was used for immunoprecipitation and ELISpot to detect Nurse shark (G. Cirratum) IgM1gj (Castro et al., 2013; PMID: 23897025). Furthermore, it was coupled to protein G beads and used in an affinity column to isolate IgM1gj from young sharks and used for IHC to detect IgM1gj in young and adult Nurse Shark spleen tissue (Castro, 2013; ProQuest: 3608068).  | 
Antibody First Published  | Castro et al. Noncoordinate expression of J-chain and Blimp-1 define nurse shark plasma cell populations during ontogeny Eur J Immunol. 2013 Nov;43(11):3061-75. doi: 10.1002/eji.201343416. Epub 2013 Aug 27 PMID:23897025  | 
Note on publication  | The original publication explores the noncoordinate expression of B-lymphocyte- induced maturation protein 1 (Blimp-1) and J-chain in nurse sharks.  | 
COA Information (For reference only, actual COA shall prevail)  | |
Size  | 100 μg Purified antibody.  | 
Concentration  | 1 mg/ml.  | 
Purification  | Protein A affinity purified  | 
Buffer  | PBS with 0.02% Proclin 300.  | 
Concentration  | 1 mg/ml.  | 
Storage Recommendation  | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.  | 

  