Anti-IgM (Igh-Cb and related haplotypes) mAbs

Cat. No.

MABL-2002

Name

Anti-IgM (Igh-Cb and   related haplotypes) mAbs

Clone No.

AFD- AF6-78.25.4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC, IF

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for IgM (Igh-Cb   and related haplotypes).

Alternative Name

IgM

UniProt

Immunogen

This antibody was raised by immunizing   C57BL/6 mice with spleen cells.

Application Notes

PI- cells were used in an flow cytometry experiment   using this antibody (Stall and Loken, 1984; pmid:6690619). B lymphoma mouse   cells were used for flow cytometry using this antibody (Alés-martinez et al,   1988; pmid:3261870). The serum concentrations of a- and b-allotype IgM,   IgG2a, and IgA were determined with ELISA using this antibody (Baumgarth et   al, 1999; pmid:10051627). Single mouse cell suspensions from spleens and PerC   were used for flow cytometery with this antibody (Baumgarth et al, 1999;   pmid:10051627). pre-B cells, B lymphocytes, plasma cells, and hybridomas from  mice were immunostained with this antibody (Velardi et al, 1984;   pmid:6432906). Serum IgM from various mouse strains was used for ELISA with   this antibody (Sieckmann et al, 1991; pmid:1709612). Single-cell suspensions   of PeC were used in a flow cytometry experiment with this antibody (Thurnheer   et al, 2003; pmid:12707334).

Antibody First   Published

Stall et al. ALLOTYPIC SPECIFICITIES OF   MURINE IgD AND IgM RECOGNIZED BY MONOCLONAL ANTIBODIES J Immunol. 1984   Feb;132(2):787-95. PMID:6690619

Note on publication

Hybridomas generated from mice immunized   with allotype and H-2-incompatible spleen cells were screened by flow   cytometry

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.