Anti-IgG mAbs

Cat. No.

MABL-1968

Name

Anti-IgG mAbs

Clone No.

AFD- NH3/130.5.2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB

Species Reactivity

rhesus macaque, Human, Cynomolgus Monkey

Basic Information

Specificity

This antibody recognises human IgG. The antibody cross-reacts with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4.

Alternative Name

immunoglobulin G; human immunoglobulin;   NH3/130

UniProt

Immunogen

This antibody was raised by immunising Balb/c   mice with whole IgG fraction and subsequent hybridoma cells formation.

Application Notes

This antibody was shown to have a high   avidity to human immunoglobulin G having a high titre, equal to a   corresponding rabbit polyclonal antibody, when tested against anti-D   sensitised cells (Downie et al., 1983). NH3/130.5.2 detects human IgG samples   transferred from the SDS polyacrylamide gel via Western blot under   non-reducing conditions (Thorpe et al., 1984). Thorne et al. (1984) mentions   that this antibody was also successful in immunoprecipitation in agar or solution (M. Spitz, unpublished results). Further, the antibody was capable of   detecting IgGs via Western blot from native gels run under acidic conditions   (Thorne et al., 1984). Finally, the antibody cross-reacted with rhesus   macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4 as shown by   ELISA analysis.

Antibody First   Published

Downie et al. The use of monoclonal   antibodies to human IgG in blood transfusion serology. Biotest Bulletin 4:   348-352 (1983)

Note on publication

This article describes the generation and   characterisation of the NH3/130.5.2 antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.