Key features and details | |
Cat. No. | MABL-1915 |
Name | Anti-I-Ab mAbs |
Clone No. | AFD- AF6-120.1.2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for the mouse Mouse I-Ab antigen. |
Alternative Name | H-2 class II histocompatibility antigen, A-B alpha chain; H2-Aa; Iaalpha |
UniProt | P14434 |
Immunogen | The antibody was raised against the mouse I-Ab antigen. |
Application Notes | Cloned lines of functionally active murine T lymphocytes derived from secondary mixed leukocyte cultures (MLC) were immunostained with this antibody (Lorber et al, 1982; pmid:6804568). Cloned lines of functionally active murine T lymphocytes derived from secondary mixed leukocyte cultures (MLC) were used in flow cytometry using this antibody (Lorber et al, 1982; pmid:6804568). Immunofluorescence was preformed on splenocytes from NOD, NON and CTS mice using this antibody (Ikegami et al, 1988; pmid:3133269). Flow cytometry was preformed using this antibody on TC-1. TC-1 is a malignant, immunogenic cell line of C57BL/6 mouse origin expressing HPV16 E6/E7 antigens (Manning et al, 2008; pmid:17725605). Cells from a p50-/- mouse were used in a flow cytometry ecperiment (Sha et al, 1995; pmid:7834752). |
Antibody First Published | Lorber et al. I-A ANTIGENS ON CLONED ALLOREACTIVE MURINE T LYMPHOCYTES ARE ACQUIRED PASSIVELY J Immunol. 1982 Jun;128(6):2798-803. PMID:6804568 |
Note on publication | Cloned lines of alloreactive cytolytic and amplifier T lymphocytes were examined for expression of I-A antigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
