Anti-HPA-1a mAbs

Cat. No.

MABL-1880

Name

Anti-HPA-1a mAbs

Clone No.

AFD- CAMTRAN007 (B2G1)

From

Recombinant Antibody

Isotype

Engineer antibody

Application

activation, Blocking, inhibition, MAIPA,   PIFT, Sensitization, ELISA, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for the leucine 33 (not the proline 33) form of human GPllla.

Alternative Name

Leu33 CD61; Platelet membrane glycoprotein IIIa leucine-33; GPIIIa Leu 33

UniProt

Immunogen

The original antibody was generated by utilizing anti-self scFv phage display library techniques.

Application Notes

The original format of this antibody (human   scFv) was in ELISA screening to test its binding to antigen-coated plates,   immunofluorescence (IF) analysis on genotyped platelets to assess binding,   and inhibition assays to evaluate its ability to block the binding of human   IgG GPIIIa leu 33-specific polyclonal alloantibodies. It showed specific   binding to GPIIb/IIIa leu 33 homozygous platelets compared to GPIIb/IIIa pro   33 homozygous platelets, as demonstrated by IF staining and   fluorescence-activated cell sorter (FACS) analysis. The purified scFv   fragments were able to inhibit the binding of polyclonal GPIIIa leu   33-specific IgG alloantibodies from plasma, resulting in a significant   reduction in binding in a Monoclonal Antibody-Specific Immobilization of Platelet   Antigen (MAIPA) assay (Griffin & Ouwehand, 1995; PMID: 8541531). 

Antibody First   Published

Griffin & Ouwehand A human monoclonal   antibody specific for the leucine- 33 (P1A1, HPA-1a) form of platelet   glycoprotein IIIa from a V gene phage display library Blood. 1995 Dec   15;86(12):4430-6. PMID:8541531

Note on publication

The original publication describes the   generation of human monoclonal antibody fragments specific for the leucine 33   form of platelet glycoprotein IIIa, which can inhibit the binding of   polyclonal human IgG alloantibodies to this form, offering potential   diagnostic and therapeutic applications for severe fetal/neonatal alloimmune   thrombocytopenia (FNAIT).

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.