Key features and details | |
Cat. No. | MABL-1870 |
Name | Anti-HLAII-DP mAbs |
Clone No. | AFD- CBL 100 BRA FB6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, IP, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for HLAII-DP. |
Alternative Name | HLA-DPB1; HLA-DP1B; MHC class II antigen DPB1; HLA class II histocompatibility antigen, DP(W4) beta chain; HLA class II histocompatibility antigen, DP beta 1 chain; MHC II; MHCII; BRA-FB6; BRAFB6 |
UniProt | P04440 |
Immunogen | The original antibody was raised by immunizing a BALB/c mouse with a non-T, non-B human acute lymphoblastic leukemia REH6 cell line. |
Application Notes | The original version of this antibody was used to immunoprecipitate HLAII-DP from the detergent lysate of radioiodinated REH6 cells (Hořejší et al., 1988; PMID:3187993). This antibody was used for immunofluorescence on B-lymphoblastoid, lymphoma and non-T, non-B leukemia cell lines (Poláková et al., 1985; PMID:3911080). This antibody was used for an ELISPOT assay on CD4+ T cells co-cultured with various stimulators (Demachi-Okamura et al., 2008; PMID:18754877). This antibody was used for flow cytometry on Hepatocytes (Guo et al., 2003; PMID:12717860), and on human PBMCs (Haylett et al., 2009; PMID:19544309). This antibody was also used in vitro on Raji cells to induce ERK1/2 activation (Haylett et al., 2009; PMID:19544309). |
Antibody First Published | Poláková et al. Monoclonal antibodies against MHC class II antigens elicited with a human non-T, non-B acute lymphoblastic leukemia cell line Neoplasma. 1985;32(6):641-8. PMID:3911080 |
Note on publication | The original paper describes a series of seven monoclonal antibodies recognizing cell surface antigens of similar distribution on a panel of leukemia/lymphoma and lymphoblastoid human cell lines was prepared by hybridoma technique after immunization with non-T, non-B acute lymphoblastic leukemia cell line REH. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
