Key features and details | |
Cat. No. | MABL-1864 |
Name | Anti-HLA-DR10 mAbs |
Clone No. | AFD- Lym-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Immunoperoxidase staining, IP, RIA, WB, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody has high affinity for a discontinuous epitope on the β subunit of the human HLA- DR10 surface membrane antigen of malignant B cells, which is not expressed on normal lymphocytes. HLA- DR10 (DR10) is a HLA-DR serotype that recognizes the DRB1*1001 gene product. |
Alternative Name | CD74; Beta chain HLA-DR; HLA-DR β-chain; β-chain; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33 |
UniProt | P04233 |
Immunogen | The original antibody was generated by immunizing mice with tumor cell nuclei preparations as immunogens. |
Application Notes | This antibody could recognize cell lines of B-cell lineage but not cells of T-cell, myeloid, or erythroid derivation in a live cell radioimmunoassay. Flow cytometric analysis of 22 lymphoma biopsies showed that the majority of B-cell tumors were LYM-1 positive. Approximately 40% of B-cell chronic lymphocytic leukemias were found to be positive with Lym-1. The binding of this antibody to peripheral blot lymphocytes was assessed using indirect immunofluorescence staining. Immunoperoxidase staining of nonlymphoid human organs and tissues revealed weak reactivity of Lym-1. This antibody was also capable of immunoprecipitating HLA-DR10 antigen from Raji cells and ARH-77 myeloma cells. (PMID: 3542194). This antibody also was used for immunohistochemical staining of advanced B-cell NHL tissues from patients (PMID: 10809213). |
Antibody First Published | Epstein et al. Two new monoclonal antibodies, Lym-1 and Lym-2, reactive with human B-lymphocytes and derived tumors, with immunodiagnostic and immunotherapeutic potential. Cancer Res. 1987 Feb 1;47(3):830-40. PMID:3542194 |
Note on publication | Describes the immunodiagnostic and immunotherapeutic potential of two monoclonal antibodies reactive with tumor derived human B cell lymphocytes. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
