Key features and details | |
Cat. No. | MABL-1838 |
Name | Anti-HIV capsid protein p24 mAbs |
Clone No. | AFD- A10F9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, WB, ELISA, IF |
Species Reactivity | HIV BJM4 |
Basic Information | |
Specificity | This antibody recognizes a continuous epitope located at α-helix 10 (residues 196 to residues 207) in the C-terminal domain of the HIV BMJ4 p24 capsid protein. The p24 capsid protein is part of the HIV- 1 Gag precursor protein (p55), together with the matrix (MA/p17) and nucleocapsid (NC/p7) protein domains. p55 is the main structural HIV-1 protein, and is uniquely responsible for virion assembly within the virus life cycle. |
Alternative Name | P24; CA; Gag polyprotein |
UniProt | Q9DGV7 |
Immunogen | The original antibody was generated by immunizing mice with p24 protein. |
Application Notes | The crystal structure of the Fab fragment in complex with the p24 protein from natural HIV-1 strain (BMJ4) was determined. The specificity of the antibody for the p 24 protein of BMJ4 strain was demonstrated by ELISA analysis. Immunofluorescence was performed on 293FT cells using this antibody (Gu et al., 2013; 23620741). The antibody detected CA N21C/A22C-V3 protein by Western blot analysis (Zhang et al. 2018; PMID: 30486318). |
Antibody First Published | Gu et al. Structure of a Novel Shoulder-to-Shoulder p24 Dimer in Complex with the Broad-Spectrum Antibody A10F9 and Its Implication in Capsid Assembly. PLoS One. 2013; 8(4): e61314. PMID:23620741 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
