Key features and details | |
Cat. No. | MABL-1803 |
Name | Anti-Heparan Sulphate Proteoglycan mAbs |
Clone No. | AFD- A7L6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IHC |
Species Reactivity | Bovine, Cod, Rat, Wolffish, Human, Mouse |
Basic Information | |
Specificity | This antibody recognizes and precipitates perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. This macromolecule has the power to store and protect growth factors, and thus plays a major rol in the control of tumour growth. |
Alternative Name | Perlecan; Perlecan proteoglycan; Heparan sulfate proteoglycan 2; Schwartz-Jampel syndrome 1; Endorepellin; Basement membrane-specific heparan sulfate proteoglycan core protein; LG3 peptide |
UniProt | P98160 |
Immunogen | The original rat IgG2a version of this antibody was raised by immunizing fisher rats with high molecular mass material from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG, and fusing the spleen cells of these rats with mouse myeloma X63 Ag8.653 cells. |
Application Notes | This original IgG2a rat clone can be used to detect and analyze the presence of perlecan. The specificity towards this protein has been confirmed using immunoprecipitation (Couchman, Ljubimov, 1989; PMID: 2477673). The perlecan was confirmed to reside in the basement membrane by means of immunofluorescence using this antibody (Couchman, Ljubimov, 1989; PMID: 2477673). Immunohistochemical staining confirmed that this clone also cross-reacts with other mammalian creatures (Couchman, Ljubimov, 1989; PMID: 2477673). In addition, one group used western blotting and immunohistochemical staining to detect significant differences in weight and distribution of antibodies across heparan sulfate proteoglycans (Tingbø et al., 2006; PMID: 16459119). |
Antibody First Published | Couchman JR, Ljubimov AV Mammalian Tissue Distribution of a Large Heparan Sulfate Proteoglycan Detected by Monoclonal Antibodies Matrix. 1989 Aug;9(4):311-21 PMID:2477673 |
Note on publication | Describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
