Anti-HA cleavage site mAbs [A3](MABL-1770)

Cat. No.

MABL-1770

Name

Anti-HA cleavage site  mAbs

Clone No.

AFD- A3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SPR, ELISA, IF

Species Reactivity

Influenza A (H5N1)

Basic Information

Specificity

The antibody specifically recognizes the sequence (NS)PQRER observed at the cleavage site of the hemagglutinin protein  of the H5N1 avian influenza virus. The antibody does not cross-react with the   HA protein of H1N1 or H7N7 strain.

Alternative Name

Hemagglutinin Cleavage Site; CS HA; Avian Influenza Virus; H5N1

UniProt

A5A5M5

Immunogen

The original antibody was generated by immunizing   mice with the cleavage site (CS) peptide of hemagglutinin protein, followed   by antibody library construction and phage display-based selection.

Application Notes

The specificity of this antibody (Fab) was   confirmed by ELISA. The epitope mapping of this antibody was done using   ELISA. The binding affinity of the Fab fragment towards the cleavage site   peptide of HA derived from the HPAI H5N1 virus was found to be Kd= 0,80 nM   using surface plasmon resonance. The clone was used for detection of the   cleavage site peptide on an open sandwich ELISA, reaching the lower limit of   detection (LOD) in the range of 1 to 10 ng/ml. This antibody was also used in   the immunofluorescent staining of Madin-Darby canine kidney cells infected   with HPAI H5N1 (Dong et al, 2013; pmid:23577205).

Antibody First   Published

Dong et al. Isolation of recombinant phage   antibodies targeting the hemagglutinin cleavage site of highly pathogenic   avian influenza virus PLoS One. 2013;8(4):e61158. PMID:23577205

Note on publication

The paper describes the generation and   characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.