Anti-H-2Kb MHC mAbs [AF6-88.5.5.3](MABL-1754)

Cat. No.

MABL-1754

Name

Anti-H-2Kb MHC mAbs

Clone No.

AFD- AF6-88.5.5.3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

inhibition, IP, FC, IF

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for mouse H-2 class   I histocompatibility antigen. H-2 class I histocompatibility antigen is   involved in the presentation of foreign antigens to the immune system.

Alternative Name

H-2 class I histocompatibility antigen, K-B   alpha chain; H-2K(B); H2-K1; H2-K

UniProt

P01901

Immunogen

This antibody was produced by the fusion of mouse myeloma Sp2/0 cells with spleen cells from a rat, that was immunized  with the H-2 class I histocompatibility antigen.

Application Notes

Cloned mouse Th cells   L2, grown on CBA/J stimulating spleen cells were used for flow cytometry   using this antibody (Wall et al, 1983; pmid:6193171). The inhibition of   125I-7 1-54.3 binding to mouse spleen cel was tested using this antibody   (Wall et al, 1983; pmid:6193171). Cells isolated from the spleen, BM, and   peripheral blood of transplanted and control animals were assayed by flow   cytometry using this antibody (Bjornson et al, 1999; pmid:9915700). Mouse   spleen leukocytes were analyzed in flow cytometry using this antibody (Xie et   al, 2006; pmid:17050600). Plenic lymphocytes derived from transgenic mice   expressing wild-type (WT) H-2Kb, ΔY or Δ7. H-2Kb were used for   immunoprecipitation. In this procedure the proteins were immunoprecipitated   using this antibody (Lizée et al, 2003; pmid:14566337). 

Antibody First   Published

Wall et al. INHIBITION OF PROLIFERATION OF   MIS- AND la-REACTIVE CLONED T CELLS BY A MONOCLONAL ANTIBODY AGAINST A   DETERMINANT SHARED BY I-A AND LE1 J Immunol. 1983 Sep;131(3):1056-64.   PMID:6193171

Note on publication

The interaction between cloned amplifier T   cells and stimulating cells was probed with the use of a monoclonal antibody  selected for its ability to inhibit stimulation of MIS- and la-reactive T   cells.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.