Key features and details | |
Cat. No. | MABL-1733 |
Name | Anti-gp120 mAbs |
Clone No. | AFD- u5.5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Neutralisation, WB, ELISA |
Species Reactivity | HIV |
Basic Information | |
Specificity | Specific to the principal neutralizing determinant (PND) of the V3 region of gp120 of HIV-1. It is reactive against peptides longer than 11 mer containing the sequence IHIGPGRAFYT (Okamoto et al, 1998). |
Alternative Name | Ru5.5; gp120; Envelope glycoprotein gp160; Env polyprotein; transmembrane protein gp41; TM gp41; glycoprotein 41; gp41; surface protein gp120; SU gp120; mu5.5 |
UniProt | U5JCI1 |
Immunogen | Mice were immunised with HTLV-IIIMN cells. Spleen cells were harvested and hybridomas generated. |
Application Notes | Has been tested against the primary field isolates of Japanese hemophiliacs seropositive for HIV-1 and protect SCID-hu mice from infection from these isolates. u5.5 was used as a humanized antibody and found to have a high neutralization activity. As of such it has been proposed as a protective antibody, however its epitope specificity gives it a limited range (Okamoto et al, 1998). It has been used in Western Blots to detect the V3 epitope of HIV-1 (Honda et al, 1995). |
Antibody First Published | D’Souza, P Monoclonal antibodies to gp160: characterization of epitopes that elicit neutralizing activity. In The Third International Workshop Antibody Serological Project The Third International Workshop Antibody Serological Project. Keystone, National Institutes of Health, Bethesda, MD., p. 25. |
Note on publication | There is no online record of this study, but it is referenced in the sequencing paper as the source of the antibody (Okamoto et al, 1998). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
