Key features and details | |
Cat. No. | MABL-1731 |
Name | Anti-GM3/GM4 terminal ganglioside sugars mAbs |
Clone No. | AFD- L612 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IA, IAA, in vivo, IP, TLC, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for gangliosides GM3 and GM4, which contain a terminal NeuAc α2-3 Galactose residue. |
Alternative Name | NeuAc α2-3 Galactose; NeuAc alpha 2-3 Galactose |
UniProt | |
Immunogen | The original antibody was generated by transforming B-lymphocytes obtained from the lymph nodes of a breast cancer patient using the Epstein-Barr virus (EBV) transformation technique. |
Application Notes | The original human IgM antibody’s reactivity was confirmed through various immunoassays, including immune adherence (IA) and immune adherence absorption (IAA) assays. It exhibited strong binding to gangliosides and effectively detected a range of human tumor tissues, such as melanoma, colon adenocarcinomas, and lung adenocarcinomas. The antibody demonstrated significant cytotoxic activity against antigen-positive tumor cells in the presence of complement. It showed notable reactivity with melanoma (62% positive) and lung cancer (100% positive) while showing less reactivity with other cancers. Neuraminidase treatment effectively inhibited its binding, affirming its specificity for gangliosides. The antibody has been used both therapeutically and diagnostically, showing efficacy in treating cutaneous malignant melanoma through intralesional injection, resulting in tumor necrosis and regression. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
