Key features and details | |
Cat. No. | MABL-1728 |
Name | Anti-GM2 mAbs |
Clone No. | AFD- DMF10.167.4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, in vivo, inhibition, ELISA, FC, IF |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | The antibody specifically binds to GM2. The epitope recognized by the antibody consists of a combination of the terminal galactose sugar and the sialic acid residue. The antibody does not cross react with GM1, GM3, sialic acid–deficient ganglioside asialoGM2. Gangliosides are glycosphingolipids that are present in high numbers on cells of neural crest origin as well as on a wide variety of tumor cells of neuroectodermal origin. |
Alternative Name | ganglioside GM2; Tay–Sachs ganglioside; β-D-GalNAc-(1→4)-[α-Neu5Ac- (2→3)]-β-D-Gal-(1→4)-β-D-Glc-(1↔1)-N-octadecanoylsphingosine |
UniProt | |
Immunogen | The original hamster antibody was raised against murine E710.2.3 thymic lymphoma cells. |
Application Notes | The antibody was employed in flow cytometry showing that numerous types of tumor cell lines expressed cell surface GM2. 14 SCLC lines were tested for surface expression of GM2 and eight lines, including LU-135, LU-134B, HTB 175, SBC-3, LU-139, HTB 171, HTB-120, and NCI-H187, showed strong positive staining for GM2 expression, while five lines, including HTB-180, NCI-H69, DMS-79, SHP-77, and HTB173, showed weak binding. CHL-1, Mel S, and Mel D, the melanoma cell lines tested, had strong surface GM2 expression. Further, two kidney carcinoma lines, HEK293 and HICK 10-4, and the Jurkat T-cell and K562 B-cell leukemia lines were strongly positive. The antibody was able to induce apoptosis and/or block cellular proliferation when cultured in vitro with the human Jurkat T lymphoma, CHL-1 melanoma, and SBC-3 small cell lung carcinoma lines. |
Antibody First Published | Retter et al. Characterization of a proapoptotic antiganglioside GM2 monoclonal antibody and evaluation of its therapeutic effect on melanoma and small cell lung carcinoma xenografts Cancer Res. 2005 Jul 15;65(14):6425-34. PMID:16024647 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
