Key features and details | |
Cat. No. | MABL-1717 |
Name | Anti-Glycoprotein D of HSV mAbs |
Clone No. | AFD- E317 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, IP, ELISA |
Species Reactivity | Herpes Simplex Virus |
Basic Information | |
Specificity | This antibody binds specifically to the glycoprotein D of HSV. The epitope core structure maps to the external surface of gD, corresponding to the binding sites of two receptors, herpesvirus entry mediator (HVEM) and nectin-1, which mediate HSV infection. E317 directly recognizes the gD-nectin-1 interface and occludes the HVEM contact site of gD to block its binding to either receptor. The binding of E317 to gD also prohibits the formation of the N-terminal hairpin of gD for HVEM recognition. The major E317-binding site on gD overlaps with either the nectin-1-binding residues or the neutralizing antigenic sites identified thus far (Tyr38, Asp215, Arg222 and Phe223). The epitopes of gD for E317 binding are highly conserved between two types of human herpesvirus (HSV-1 and HSV-2). |
Alternative Name | Envelope glycoprotein D; gD |
UniProt | Q69091 |
Immunogen | Herpes simplex virus 1. |
Application Notes | This antibody binds to glycoprotein D of HSV and blocks its intereaction with human cellular receptors. Glycoprotein D binds to cell entry receptors TNFRSF14/HVEM and others, triggering fusion with the host membrane. |
Antibody First Published | Lee et al. Structural basis for the antibody neutralization of herpes simplex virus. Acta Cystallography Section D Biological Crystallography 2013; 69(Pt19):1935-1945 PMID:24100313 |
Note on publication | Describes the generation of a monoclonal antibody against HSV glycoprotein D and subsequent crystallisation as well as a discussion of its potential therapeutic use. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
