Anti-Glycophorin A M antigen mAbs

Cat. No.

MABL-1711

Name

Anti-Glycophorin A M antigen mAbs

Clone No.

AFD- M2A1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

immunoblot, inhibition assay, ELISA

Species Reactivity

Human

Basic Information

Specificity

M2A1 binds specifically to the M antigen on   human erythrocytes, and shows no reactivity to N antigen. The epitope for   this antibody includes the amino-terminal serine residue and sialic acid  residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and   salt-dependent (optimum ~ pH8-9). Blood group antigen M is located on the   major sialoglycoprotein (glycophorin A) of red blood cells.

Alternative Name

CD235a; M sialoglycoprotein; PAS-2

UniProt

P02724

Immunogen

M2A1 was prepared by immunzing BALB/c mice with human red blood cells of the M phenotype, and was screened by testing for anti-M activity.

Application Notes

M2A1 can bind to M antigen of glycophorin A and can be used in ELISAs, immunoblots and modified M glycoprotein can be used   for inhibition tests.

Antibody First   Published

Jaskiewicz et al. Characterization of the   epitope recognized by a monoclonal antibody highly specific for blood group M   antigen. Transfusion. 1990 Mar-Apr;30(3)：230-5. PMID：1690467

Note on publication

Describes the generation and   characterization of M2A1 antibody against M antigen.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.