Key features and details | |
Cat. No. | MABL-1708 |
Name | Anti-glutathione-S-transferase (GST) mAbs |
Clone No. | AFD- 1C9-B4-A2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for both native and denatured GST protein. GST protein is a protein Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds. |
Alternative Name | Glutathione S-transferase alpha-1; Gsta1; 13- hydroperoxyoctadecadienoate peroxidase; Androst-5-ene-3,17-dione isomerase; GST 1-1; GST 1a-1a; GST A1-1; GST B; Glutathione S-transferase Ya-1; GST Ya1; Ligandin; GST |
UniProt | P00502 |
Immunogen | The mouse version of this antibody was generated by immunizing BALB/c mice with GST-9.11 fusion protein. |
Application Notes | To make sure fusion proteins were fused to the plastic surface the mouse version of this antibody was used in an ELISA (Ramos and DeSimone, 1996; pmid：8698817). An ELISA was preformed on petri dishes coated with fusion proteins to confirm the absorption of the proteins to the petri dish (Ramos et al, 1996; pmid：8787760). |
Antibody First Published | Ramos and DeSimone. Xenopus embryonic cell adhesion to fibronectin: position-specific activation of RGD/synergy site-dependent migratory behavior at gastrulation J Cell Biol. 1996 Jul;134(1)：227-40. PMID：8698817 |
Note on publication | Regions of FN required for the attachment and migration of involuting marginal zone (IMZ) cells were analyzed in vitro using FN fusion protein substrates. IMZ cell attachment to FN is mediated by the Arg-Gly-Asp (RGD) sequence located in the type III-10 repeat and by the Pro-Pro-Arg-Arg-Ala-Arg (PPRRAR) sequence in the type III-13 repeat of the Hep II domain. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
