Key features and details | |
Cat. No. | MABL-1705 |
Name | Anti-Glomerular antigen mAbs |
Clone No. | AFD- G1-12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody displays polyreactive binding to GA, ssDNA, dsDNA and IgG. It does not bind histones or Sm proteins. |
Alternative Name | GA |
UniProt | |
Immunogen | This antibody was generated from renal mononuclear, Ab-producing cells (AbPCs) derived from diseased MRL/pr mouse kidneys. MRL/pr mice spontanously develop an automimmune syndrome associated with autoantibody production, and act as models of human systemic lupus erythematosus (SLE). |
Application Notes | The binding specificity of this antibody has been confirmed in ELISA and Western blot analysis (Sekine et al, 2004). Intravenous administration of this antibody to normal mice results in glomerular binding, as revealed by immunofluoescence analysis, suggesting that the production of glomerular-binding autoantibodies by renal mononuclear cells conributes to the developmet of lupus nephritis (Sekine et al, 2004). |
Antibody First Published | Sekine et al. Enrichment of anti-glomerular antigen antibody-producing cells in the kidneys of MRL/MpJ-Fas(lpr) mice. J Immunol. 2004 Mar 15;172(6)：3913-21. PMID：15004199 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
