Key features and details | |
Cat. No. | MABL-1690 |
Name | Anti-gHgL mAbs |
Clone No. | AFD- CL59 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralizing, WB, ELISA, FC |
Species Reactivity | Epstein-Barr virus |
Basic Information | |
Specificity | The antibody binds an epitope in gH D-IV, proximal to the viral membrane and distant from known receptor binding sites. In particular, the antibody binds to the back side of gH D-III/D-IV close to gH D- III residues 494–503 (loop between 3α-8 and 3α-9), 456–468 (3α-6 helix), and 406–415 (loop between 3α-3 and 3α-4), and gH D-IV loop residues 645–656 (loop between 4α-1 and 4β-9), 623–626 (loop between 4β-7 and 4β-8), and 568–577 (loop between 4β-3 and 4β-4). |
Alternative Name | Envelope glycoprotein H; Envelope glycoprotein L |
UniProt | P03231; P03212 |
Immunogen | |
Application Notes | The antibody blocks EBV-mediated membrane fusion with both B cells and epithelial cells. Flow cytometry cell counts showed that Fab fragment inhibits virus entry. Neutralization experiments with epithelial cells using an EBV-GFP reporter virus and purified Fab. Fluorescence images obtained at 24 h postinfection and flow cytometry cell counts showed that the Fab inhibits virus entry. The Fab exhibited ∼50% inhibition of EBV infection at 150 nM, where no inhibition of cell–cell membrane fusion was observed. (PMID： 28939750) The cell-surface expression of gHgL was evaluated using the antibody by CELISA. (PMID： 27929061) |
Antibody First Published | Sathiyamoorthy et al. Inhibition of EBV-mediated membrane fusion by anti- gHgL antibodies. Proc Natl Acad Sci U S A. 2017 Oct 10;114(41)：E8703-E8710. PMID：28939750 |
Note on publication | The authors studied the structure and mechanism of anti-gHgL antibody CL59 that blocks membrane fusion with both B cells and epithelial cells. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
