Key features and details | |
Cat. No. | MABL-1672 |
Name | Anti-Genome polyprotein mAbs |
Clone No. | AFD- PVSP6A |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, neutralization, SPR |
Species Reactivity | Poliovirus |
Basic Information | |
Specificity | This antibody is specific for Capsid proteins VP1 (GH loop, EF loop and C beta strand) and VP2 (EF loop) of the polio genome polyprotein. |
Alternative Name | Capsid protein VP1; Capsid protein VP2; Poliovirus type 1 |
UniProt | P03300 |
Immunogen | The original antibody was raised by immunizing a dromedary with poliovirus type 1 Sabin strain. |
Application Notes | The binding strength of the original antibody to poliovirus antigens was determined using surface plasmon resonance(Thys et al., 2011; PMID: 21371506). The original antibody was used for a neutralization assay against three types of poliovirus. This antibody showed strong neutralization in type 1 polioviruses. The cytotoxicity of this antibody on HeLa cells was also evaluated. It showed no cytotoxicity (Thys et al., 2010; PMID：20566349). The effect of this antibody on virus entry and uncoating was determined. It showed change in the behavior of the polio particles (Schotte et al., 2014; PMID：24501405). The structure of this antibody was determined using cryo-EM (Strauss et al., 2016; PMID：26764003). |
Antibody First Published | Thys et al. In vitro antiviral activity of single domain antibody fragments against poliovirus Antiviral Res. 2010 Aug;87(2)：257-64 PMID：20566349 |
Note on publication | The original paper aimed to determine whether VHHs inhibit wild-type and vaccine strains of poliovirus type 1. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
