Anti-gelatinase B mAbs

Cat. No.

MABL-1667

Name

Anti-gelatinase B mAbs

Clone No.

AFD- REGA-3G12

From

Recombinant Antibody

Isotype

Engineer antibody

Application

immunoblot, inhibition, IP, WB, ELISA

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human gelatinase B; it recognizes the catalytic site region between Trp116 and Lys214 of the enzyme's globular aminoterminal part. A dimer scFv (with a His6   tag) variant of this antibody was found to cross-react with gelatinase A.

Alternative Name

GB; GELB; MMP 9; MMP9; MMP-9; gelatinase MMP   9; 92-kDa gelatinase; matrix metalloproteinase 9; Matrix metalloproteinase-9;   neutrophil gelatinase; natural human neutrophil gelatinase B; type V   collagenase; 92-kDa type IV collagenase; macrophage gelatinase; 95 kDa type   IV collagenase/gelatinase; collagenase IV; collagenase type IV; type IV collagen metalloproteinase; 3G12; 3G12T; 3G12 scFv; 3G12scFv

UniProt

P14780

Immunogen

The original antibody was generated by immunizing Balb/c mice with a purified gelatinase B enzyme preparation.

Application Notes

The specificity of this   antibody's original format (mouse IgG1, κ) was evaluated by ELISA; it   specifically bound to gelatinase B with a binding affinity of 2.1 x 10-9 M.   This antibody detected both native and H2NPhAcHg-treated gelatinase B in an   immunoblot, both in non-reducing and reducing conditions. Furthermore, this   antibody was found to inhibit gelatinase B activity in vitro (Paemen et al.,   1995; PMID: 8575432). The binding of the scFv version of this antibody to   gelatinase B was confirmed in an ELISA. The original antibody and its F(ab')2   and scFv derivatives inhibited gelatinase B in vitro (Zhou et al., 1997;   PMID: 9323036). The following scFv derivatives of this antibody were   evaluated for their inhibitory effect toward gelatinase B: REGA-3G12 scFv   with (His6) monomer (10 μM), REGA-3G12 scFv with (His6) dimer (5 μM), and REGA-3G12 scFv without a tag (1.25, 2.5, and 5 μM). They achieved 6.5%,   65.9%, 8.8%, 32.4%, and 44.4% inhibitory activity, respectively. 

Antibody First   Published

Paemen et al. Monoclonal   antibodies specific for natural human neutrophil gelatinase B used for   affinity purification, quantitation by two-site ELISA and inhibition of   enzymatic activity Eur J Biochem. 1995 Dec 15;234(3):759-65. doi:   10.1111/j.1432-1033.1995.759_a.x PMID:8575432

Note on publication

The original publication   focuses on the production and characterization of mouse monoclonal antibodies   specific for natural human neutrophil gelatinase B.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.