Key features and details | |
Cat. No. | MABL-1644 |
Name | Anti-Galectin 9 mAbs |
Clone No. | AFD- 1G3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, functional assays, neutralization, WB, ELISA, IHC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for human Gal-9 but exhibits cross-binding of murine Gal-9. |
Alternative Name | Galectin-9; Gal-9; LGALS9; Ecalectin; Tumor antigen HOM-HD-21 |
UniProt | O00182 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a recombinant c- terminus galectin-9 (residues 191 to 355 of the galectin-9 long isoform). |
Application Notes | The binding and binding specificity of the original format of this antibody to c-terminus Gal-9 were assessed via ELISA. Epitope mapping was also done using ELISA. Furthermore, this antibody was successfully used in WB and IHC assays (Barjon et al., 2012; PMID: 22805533). An scFv-CAR version of this antibody with therapeutic potential was developed and described (US20230295564A1). This antibody was successfully used to neutralize LGALS9 in a study focused on pancreatic neoplastic lesions (Quilbe et al., 2023; PMID: 38098486). This antibody was used in a T lymphocyte proliferation assay, specifically in assessing the immunomodulatory properties of extracellular vesicles (C15SEVs) derived from nasopharyngeal carcinoma and recombinant Galectin-9S. In this assay, the antibody was used to block the impact of C15SEVs and recombinant Galectin-9 on peripheral blood mononuclear cells (Lefebvre et al., 2023; PMID: 38117000). |
Antibody First Published | Barjon et al. A novel monoclonal antibody for detection of galectin-9 in tissue sections: application to human tissues infected by oncogenic viruses. Infect Agent Cancer. 2012 Jul 17;7(1):16. PMID:22805533 |
Note on publication | The original publication presents the development of monoclonal antibodies targeting galectin-9 and focuses on the development and characterization of a novel monoclonal antibody, 1G3, with a particular emphasis on its application in improving the detection and understanding of galectin- 9 expression in clinical samples. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
