Anti-Frizzled mAbs

Cat. No.

MABL-1625

Name

Anti-Frizzled mAbs

Clone No.

AFD- 1C11-B9-H9-G9-D9

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IF, IHC

Species Reactivity

Drosophila melanogaster

Basic Information

Specificity

This antibody is specific for drosophila frizzled. It is specific for amino acids 1-252. Frizzled is a receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin   canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin   and activation of Wnt target genes.

Alternative Name

Frizzled protein; fz; Frizzled-1; dFz1

UniProt

P18537

Immunogen

The mouse version of this antibody was generated by immunizing a mouse with amino acids 1-252 of Drosophila Frizzled.

Application Notes

To study the biochemical characteristics of   the frizzled protein. Proteins from schneider 2 cells were immunoprecipitated  using the mouse version of this antibody. Futhermore the precipitates were   immunoblotted using this antibody. Finally both schneider cells and pupal   wings were immunostained using this antibody (Park et al, 1994;  pmid:8199049). Protein lysates from drosophila L cells were immunoblotted using the mouse version of this antibody (Pradhan-Sundd and Verheyen, 2015;   pmid:26224310). Adult drosophila wings were immunostained using the mouse   version of this antibody (Adler et al, 1997; pmid:9382848). To determine the   link between the wingless and the frizzled proteins. A immunolabeling was  preformed on drosophila embryos using this antibody (Müller et al, 1999;   pmid:9876186). To understand the wingless pathway. P19 cell lysates were   immunoblotted using this antibody (Abdul-Ghani et al, 2011; pmid:21041481).

Antibody First   Published

Park et al. The frizzled gene of Drosophila   encodes a membrane protein with an odd number of transmembrane domains Mech   Dev. 1994 Feb;45(2):127-37. PMID:8199049

Note on publication

To study the biochemical characteristics of   this rare protein, we constructed a hs-fz fusion gene and transferred this to   Drosophila tissue culture cells and embryos. Cell fractionation experiments   and immunostaining experiments showed that the Fz protein is an integral   membrane protein containing an odd number of transmembrane domains,   consistent with previous suggestions that it contains seven transmembrane   domains.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.