Anti-FlgI mAbs

Cat. No.

MABL-1609

Name

Anti-FlgI mAbs

Clone No.

AFD-932

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Liberibacter asiaticus

Basic Information

Specificity

Clone 932 was shown to bind purified FlgI by ELISA (Yuan et al, 2015).

Alternative Name

Flagellar P-ring protein; Basal body P-ring   protein

UniProt

C6XHV6

Immunogen

BALB/c mice were immunised by injection of a high titre of Liberibacter asiaticus obtained from infected Diaphorina citri  psyllids. The spleens of immunised mice were removed and homogenised for RNA   extraction and cDNA synthesis.

Application Notes

Clone 932 was tested for Liberibacter   asiaticus recognition as a phage-expressing scFv using blotting (Yuan et al,   2015). This format was also tested against specific recognition of the   antigen FlgI using ELISA.

Antibody First   Published

Yuan et al. Development of single chain   variable fragment (scFv) antibodies against surface proteins of 'Ca.   Liberibacter asiaticus'. J Microbiol Methods. 2016 Mar;122:1-7. PMID:26744234

Note on publication

Describes the generation and brief   characterisation of anti-Liberibacter asiaticus scFv-formatted antibody   fragments specific for different antigens.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.