Key features and details | |
Cat. No. | MABL-1600 |
Name | Anti-Fibronectin EIIIB mAbs |
Clone No. | AFD- NJB2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunoblot, in vivo, ELISA, IF, IHC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for the FN-EIIIB splice variant expressed in the ECM of tumors, metastases, and other diseases characterized by ECM deposition. |
Alternative Name | FN-EIIIB; EDB domain of fibronectin |
UniProt | |
Immunogen | The original antibody was isolated from an alpaca-derived phage display library, by panning against the EIIIB domain of fibronectin. |
Application Notes | This nanobody was shown to detect primary tumors and metastatic sites in vivo by specifically binding to the ECM with excellent specificity in multiple models of breast cancer, including human and mouse triple-negative breast cancer, and in melanoma, as measured by PET/CT imaging with 64 Cu-NJB2. This nanobody recognized EIIIB-His by ELISA and in FN-containing EIIIB immunohistochemistry (IHC). Furthermore, its binding affinity to immobilized single EIIIB domain and a large recombinant rat FN fragment comprising FNIII7–15, including EIIIB (FN 7–15 EIIIB), was 1.9 × 10-9 M and 2.25 × 10-9 M, respectively, as determined by biolayer interferometry (BLI) (Jailkhani et al., 2019; PMID: 31068469). This nanobody was used for IHC of B16F10 and MC38 tumors to detect EIIIB expression (Xie et al., 2019; PMID: 30936321). |
Antibody First Published | Jailkhani et al. Noninvasive imaging of tumor progression, metastasis, and fibrosis using a nanobody targeting the extracellular matrix Proc Natl Acad Sci U S A. 2019 Jul 9;116(28):14181-14190. doi: 10.1073/pnas.1817442116 PMID:31068469 |
Note on publication | The original publication presents the development and application of NJB2, a nanobody that targets disease-associated extracellular matrix, enabling noninvasive imaging of tumors, metastases, and fibrosis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
